4th Ligation Day

Orléans is located 110 km north of Paris. There is regular trains from Paris Austerlitz station and from Tours train station (both around 1 h travel), as well as direct shuttles from the two Paris airports (Orly and Roissy-Charles de Gaulles).

The conference venue is unique. Located right next to the Orléans’ cathedral, the episcopal palace of Orléans, built between 1635 and 1641, locally known as the Hôtel Dupanloup, is a classical French building which served until 1905 of residence to the bishops of Orléans.  Since 2014, the renewed palace hosts the International University Center for Research and Le Studium Loire Valley Institute for Advanced Studies.

Participants will be welcomed in this exceptional surrounding, blending Middle Age and Renaissance cultures with modern design and will have the opportunity to discover French cuisine and wines.

Venue adress: 1, rue Dupanloup – 45000 ORLEANS – FR

Hôtel Dupanloup is 10 min walk from Orléans train station. Alternatively, you can take tram A to De Gaulle station then tram B to Cathédrale-Hôtel de Ville station.

List of recommended hotels in Orléans  

Organizing committee:

• Dr Vincent AUCAGNE
• Dr Vishwanatha THIMMALAPURA MARULAPPA

• Sophie GABILLET, General Secretary
• Dr Aurélien MONTAGU, Scientific Relations Manager
• Maurine VILLIERS, Events Projects Officer

Scientific committee:

• Dr Vincent AUCAGNE
• Dr Agnès DELMAS
• Dr Vishwanatha THIMMALAPURA MARULAPPA

CNRS Center for Molecular Biophysics (CBM), Orléans, France

Click here to register to the 4^th Chemical Ligation Meeting

Please note that participation is limited to 120 people. Registration will be on a first-come, first-serve basis.

Key Dates:

November 18^th, 2019 : deadline for oral communication submission

December 2^nd, 2019 : deadline for poster submission. January 20^th, 2020 : end of registration.  

Registration fees include :

Deadline for submitting an abstract for oral communication ended on November 18th, 2019, and the oral communication selection process is now over (see the selected oral communications at the bottom of the page) .

Deadline for submitting an abstract for poster communication has been postponed to January 17^th, 2020

Please upload your abstract during the registration or send it before the deadline to . 

Note that we do not print the posters, but racks & pins will be provided for up to A0 sizes, portrait format.

Please download the template for poster presentation.    

List of the oral communications selected from the submitted abstracts:

MONDAY JANUARY 27^th 2020

• 12:30    Welcome lunch and registration
• 14:00    Official opening
• 14:20    Prof. Matthew Francis – Protein-Protein Coupling Using Enzymatic Oxidative Coupling Reactions
• 15:00    Dr Sébastien Gouin – From the design of an antibacterial glycoconjugate to the development of a chemoselective bioconjugation method for tyrosine.
• 15:20    Dr Elisabetta Mileo – Nitroxide-based spin tags coupled to EPR spectroscopy: investigation of protein structural dynamics in vitro and inside cells
• 15:40    Ruben Tomás – Controlling Synthetic Polymer Ligation to Metabolically Labelled Cellular Interfaces to Introduce Non-native Functionality
• 15:50    Coffee Break + Poster session 1
• 16:30    Dr Vladimir Torbeev – Making order out of disordered proteins
• 17:00    Dr Olivier Sénèque – How chemoselective ligations make the design of peptide-based responsive luminescent probes easier
• 17:30    Dr Somnath Mukherjee – Impact of site-specific non-enzymatic posttranslational modification on the structure-activity attributes of human Heat Shock Protein 27 (Hsp27)
• 17:50    Dr Elisabeth Garanger – Expanding the toolbox of chemoselective modifications of protein-like polymers at methionyl residues
• 18:00    Coffee Break
• 18:30     Public lecture in French: Dr Denis Servent – Venins, du poison au médicament

TUESDAY JANUARY 28^th 2020

• 08:45    Welcome coffee
• 09:00    Prof. Lutz Ackermann – C─H Activation as an Transformative Tool for Late-stage Diversification of Biomolecules
• 09:40    Dr Samir Messaoudi – Synthesis of aryl-thioglycopeptides through chemoselective Pd-mediated conjugation
• 10:00    Rebecca Schäfer – The Bioorthogonal Isonitrile-Chlorooxime Ligation
• 10:10    Coffee Break + Poster session 2
• 11:10    Dr Didier Boturyn – Design of RGD peptide conjugates for imaging and therapeutic applications
• 11:50    Prof. Beat Fierz – Synthetic and single-molecule exploration of the dynamic chromatin landscape
• 12:20    Lunch
• 13:30    Dr Frédéric Taran – Recent advances in fast, bioorthogonal ligation reactions
• 14:10    Dr Cyrille Sabot – From organic methodology to the development of chemoselective ligations
• 14:40    Dr Laia Miret Casals – Furan crosslink technology: from in vitro analysis of peptide-protein interactions to GPCR-ligand interactions on live cells
• 15:00    Dr Pierre Milbeo – Development of Novel Thiol-ene Mediated Peptide Ligation Strategies
• 15:20    Dr Gosuke Hayashi – Chemical synthesis of histone proteins through novel ligation strategies
• 15:40    Coffee Break + Poster session 3
• 16:20    Prof. Carlo Unverzagt – Merging Chemistries for the Synthesis and Evaluation of Homogenous Glycoproteins
• 17:00   Dr Kevin Neumann – Cyanosulfurylide as a protecting group for carboxylic acid during peptide synthesis
• 17:20    Prof. Wenshe Liu – Phage-Encoded Noncanonical Amino Acids for Drug Discovery
• 17:40    Guided visit of Orléans town center

WEDNESDAY JANUARY 29^th 2020

• 08:45    Welcome coffee
• 09:00    Dr Oleg Melnyk – A cysteine selenosulfide redox switch for protein chemical synthesis
• 09:40    Dr Fabienne Burlina – Extending chemical ligation for the synthesis of proteins: Application to NEMO ubiquitylation
• 10:10   Dr Marina Rubini – Semisynthetic approaches for studying post-translational modifications
• 10:30    Coffee Break
• 11:00    Dr Barbara Bernardim – Title to be announced
• 11:20    Prof. Alexander Dömling – Multicomponent reaction chemistry: From novel small molecules to automated nano chemistry to stapled peptides
• 12:00    Official closing
• 12:10     Farewell cocktail

The symposium entitled:

Challenges and prospects in chemoselective ligations: from protein synthesis to site-specific conjugation

have been held in Orléans (France), from Monday 27th to Wednesday 29^th January 2020.

This three-day international conference was jointly organized by LE STUDIUM Loire Valley Institute for Advanced Studies and the Centre for Molecular Biophysics – CNRS Orléans. This event was held under the auspices of the French Peptide and Protein Group (GFPP) and followed three previous editions (Paris 2001, Lille 2008 and 2016) of short international meetings dedicated to chemical ligation. The official language of this meeting is English.

Recent years have seen an exponentially growing interest in chemical reactions enabling the selective modification of proteins and peptides. These advances are intimately linked to the development of methodologies for protein engineering with an atomic precision, for the generation of well-defined antibody-drug conjugates or on-demand probes to decipher biological processes. Such chemoselective reactions are also widely utilized for the total synthesis and semi-synthesis of proteins through chemical ligation of unprotected peptide fragments, an approach highly complementary to recombinant production. The chemical reactions implemented for both purposes share the same requirements: compatibility with dilute aqueous solution at physiological pH, and tolerance to the myriad functional groups commonly found in biomolecules. These remarkable features inspired the advent of bioorthogonal reactions, which can be carried out in living systems.

Sixteen invited speakers have accepted our invitation and covered the different aspects of these multidisciplinary fields, from methodology development to biological applications. In addition, contributions selected from submitted abstracts have been presented as oral communication and poster.